![]() The QuickPick™ DNA Fragment kit provides a fast and simple means of isolating DNA fragments from gel or from liquid solutions. The kit and Bio-Nobile's PickO™ gel excisers are suitable for use with standard and low-melting agarose gels in TAE or TBE buffer. The technique does not require organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. Purified DNA fragments can be used for downstream applications such as automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription/translation, restriction mapping, restriction enzyme digestion, cloning and labelling. metal affinity kit for proteins, 48 tal affinity kit for proteins, 8 preps. The QuickPick IMAC kits provide a fast and simple means for recombinant histidine-tagged (His-tag) protein purification based on metal affinity using Ni 2+. Immobilized metal ion affinity chromatography (IMAC) was originally introduced as a method for group separation, but today it represents one of the most important tools for the single-step purification of proteins based on polyhistidine tags. The principle of the mechanism is based on the interaction between a metal ion coordinated to a covalently bound chelating ligand and a His-tag protein. The QuickPick™ CM and DEAE kits are based on magnetic cellulose particles coated with carboxymethyl (CM) groups or diethylaminoethyl (DEAE) groups. Different types of proteins will bind to the coated magnetic particles with affinities that depend on both the conditions used and the types and number of individual charged groups. ![]()
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